Monday, July 6th, 2020


Gentian Violet Used as an Epithelial Cell Monolayer Stain in the Scratch Wound Healing Assay
Authors:  César Rivera, B.Dent.Sc., D.D.S., M.Bio.Sc.
  To the Editors: Cell migration plays a major role in development, physiology, and pathology and is frequently evaluated in vitro by the scratch wound healing assay (WHA),1 an easy, low-cost, and well-developed method. The steps involve (1) creating a “scratch” in a cell monolayer, (2) capturing the images at regular intervals during cell migration to close the scratch, and (3) comparing the captures to quantify the migration rate of the cells.2 The experimental conditions can be adjusted easily for different purposes. This letter presents a gentian violet (GV) staining technique combined with conventional light microscope for cell scratch analysis.
Keywords:  crystal violet, epithelial cells, gentian violet, methyl violet, scratch-wound healing assay
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