Thursday, June 21st, 2018


Agarose Floor Technique: A Simple Scaffold-Free Method for 3D Cell Culture and Multicellular Tumor Spheroid Formation
Authors:  C├ęsar Rivera, B.Dent.Sc., D.D.S., M.Sc.
  To the Editors: 3D cell cultures can close the gap between in vitro experiments used for discovery and in vivo designs used for efficacy and safety assessment before proceeding to clinical studies.1 3D multicellular tumor spheroids better reflect the tumor environment in terms of phenotypic heterogeneity, nutrient and oxygen gradients, intervascular domains, and micrometastases.2 This letter presents the agarose floor technique to obtain single-cell suspensions from oral cancer cells in nonadherent conditions. SCC-9 cells (American Type Culture Collection, Manassas, Virginia, USA) were cultured in culture plastic wares with a nonadhesive surface.
Keywords:  agarose, three-dimensional tumor spheroids, tumor spheroid models
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