Wednesday, May 23rd, 2018

 

Next
A full text version of this article is available.
To access article obtain online access here or login
 
Title:
Microwave Tissue Dehydration: A Fast and Reliable Tissue-Processing Method in Pathology
Authors:  Yanwei Li, M.M., Shaoshuai Mei, B.M., Yayan Cui, M.D., Fei Teng, M.M., and Wei Wang, B.M.
  Objective: To compare the histologic quality of tissues processed with rapid microwave tissue dehydration and traditional tissue dehydration.
Study Design:
A total of 200 paired tissues from 15 organs were taken and divided into 2 groups. One group was processed with rapid microwave tissue de- hydration, and the other one was processed with traditional tissue dehydration. Hematoxylin and eosin (H&E), immunohistochemical (IHC), and in situ hybridization (ISH) staining were conducted, and the staining qualities were compared carefully. Evaluation of blocks, H&E stain, IHC stain, and ISH were carried out.
Results:
The quality of blocks and H&E stain showed no distinction. The IHC signal-positive location, percentage of positive cells, grades of intensity, and the staining background showed a very high concordance between the 2 groups. The ISH results show no distinction from each other. Moreover, microwave tissue dehydration is time-saving.
Conclusion:
As a rapid and reliable tissue processing method, microwave tissue dehydration may potentially be useful when rapid pathological diagnosis is needed.
Keywords:  conventional tissue processing, histology, histotechnology, immunohistochemistry, microwave tissue dehydration, rapid tissue processing, staining, tissue stains, tissue
   
   
  Acrobat Reader 7.0 is recommended to properly view and print the article.
Reader can be downloaded from