Friday, February 22nd, 2019


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Multifocal Clonal Evolution Characterized Using Circulating Tumor DNA Compared with Tumor Biopsies in Metastatic Colorectal Cancer by Targeted Next Generation Sequencing
Authors:  Lei Li, Ph.D., Haitao Chen, B.S., Linlin Chai, M.M., Lin Zou, M.M., Juanjuan Xu, M.M., Xiaodan Cheng, M.M., Jianyun Lan, M.M., and Wenzhang Zha, M.M.
  Objective: Intratumor clonal heterogeneity limits efficacy and duration of response to targeted treatments in metastatic cancer. About 50% of colorectal cancer patients have such distant metastases, accounting for virtually all deaths from the disease. We followed a patient with metastatic colon carcinoma who had undergone several surgeries after being diagnosed with colorectal cancer in 2006 and had survived for over 10 years.
Study Design:
We characterized 5 tumor biopsies and 1 plasma sample collected at clinical follow-up using high throughput sequencing.
Serial changes in circulating levels of subclonal private mutations correlated with different treatment responses between metastatic sites.
This comparison of biopsy and plasma sample in a single patient with metastatic colorectal cancer shows that circulating tumor DNA can allow real-time sampling of multifocal clonal evolution. Through our research, we hope the information of the mutations in this patient would assist studying the relationship between mutations and survival.
Keywords:  colorectal cancer, tumor biopsies, tumor DNA
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