Thursday, February 27th, 2020


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Dihydrotanshinone I Induces Apoptosis Through Reactive Oxygen Species–Mediated Oxidative Stress in AGS Human Gastric Cancer Cells
Authors:  Xiongdong Zhong, M.Sc., Ni Gu, M.Sc., Lei Chen, B.Sc., and Yunlong Pan, Ph.D.
  Objective: To investigate the anticancer actions of dihydrotanshinone I (DHTS) in AGS human gastric cancer cells.
Study Design:
Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Intracellular reactive oxygen spe-cies (ROS) levels were determined using flow cytometry. Caspase activities were measured with fluorometric assay.
Results from MTT assay showed that DHTS significantly inhibited AGS cell viability in dose- and time-dependent manners. Elevated intracellular ROS levels and increased apoptotic cells were observed in DHTS-treated AGS cells. In addition, activation of caspase-3 and caspase-8, rather than caspase-9, was no- ticed in DHTS-treated AGS cells. Furthermore, block-ing ROS generation with N-acetylcysteine markedly reversed DHTS-induced cell apoptosis.
All the findings strongly suggest that DHTS can initiate ROS generation and induce oxidative stress and cell apoptosis in AGS human gastric cancer cells, which deserves to be further developed as an anticancer agent.
Keywords:  alternative medicine; apoptosis; apoptosis/drug effects; cell line, tumor; cell proliferation/drug effects; complementary therapies; Danshen; dihydrotanshinone I; gastric cancer; humans; mitogen-activated protein kinases/metabolism; reactive oxygen species; Salvia miltiorrhiza
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