Wednesday, April 21st, 2021


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Effects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model
Authors:  Nilgün Sögütçü, M.D., and Beran Yokus, Ph.D.
  Objective: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion.
Study Design:
Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion+gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720° clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion+gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-α antibody.
MDA levels in the torsion/detorsion+gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion+gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion+gallic acid group. TNF-α expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion+gallic acid group, TNF-α expression was observed in some interstitial region rather than in cells of seminiferous tubules.
Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis.
Keywords:  detorsion; ischemia-reperfusion injury; oxidative stress; rats, Wistar; reperfusion injury; spermatic cord torsion; spermatogenesis; testicular torsion; testis; torsion abnormality; torsion-detorsion
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