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Title:
Effects of miRNA-34c on the Proliferation and Migration of Ovarian Epithelial Cancer Cells by Targeting E2F3
Authors:  Sisi Zhang, M.M., Li Xiao, M.D., Zheng e Zhang, M.M., and Shuirong Zhang, M.M.
  Objective: To explore the effect of microRNA (miRNA)-34c on the proliferation of ovarian epithelial cancer cells (OV-1063) by regulating the expression of E2F3.
Study Design:
RT-PCR was used to detect the expression level of miRNA-34c mRNA in ovarian epithelial tissues and ovarian epithelial cancer tissues. OV-1063 cells were transfected by miRNA-34 MIMIC (miRNA-34c analog), and transfection was detected by RT-PCR. After OV-1063 cell experiment group and OV-1063 cell control group miRNA-34c 24 hour, 48 hour, and 72 hour relative expression of mRNA to test whether OV-1063 cell transfection was successful. After successful transfection, MMT method was used to detect OV-1063 cell proliferation ability. Transwell assay was used to detect OV-1063 cell invasion ability. Western blot method was used to detect OV-1063 cell E2F3 protein expression level.
Results:
Compared with ovarian epithelial tissues, the expression of miRNA-34 in ovarian epithelial cancer tissues was significantly downregulated (p<0.01); compared with the control group, there was no difference in the proliferation ability of OV-1063 cells 24 hours after transfection (p>0.05). After 48 hours and 72 hours, the proliferation ability of OV-1063 cells was significantly weaker than that of the control group (p<0.01). The invasion ability of OV-1063 cells after transfection was significantly lower than that of the control group (p<0.01). E2F3 in OV-1063 cells after transfection MRNA and protein expression levels were significantly lower than the control group (p<0.01).
Conclusion:
miRNA-34c can reduce the proliferation and invasion of ovarian epithelial cancer cells by downregulating the expression of E2F3, thereby exerting an anti-tumor effect. MiRNA-34c and E2F3 may be potential therapeutic targets for ovarian epithelial cancer.
Keywords:  E2F3, epithelial ovarian cancer, miRNA-34c, ovarian cancer
   
   
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