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Title: |
Study on the Coagulation Function of Frozen Platelets in Vitro by Thromboelastography | |||||||||||||||||||
| Authors: | Xinwei Shi, M.M., Yaoqiang Du, M.M., Changchang Chen, and Jian Shen, M.M. | |||||||||||||||||||
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Objective: To compare the coagulation function of frozen and fresh platelets in vitro, and investigate the effect of shock storage or cryopreservation on platelet function.
Study Design: Platelets of healthy people were collected, divided into 2 equal parts, respectively, and subjected to shock storage and cryopreservation. Using 5% DMSO as protective agent, platelets were frozen at −80°C low temperature, then stored for 4 days and cryopreserved for 4 days. The MA value was detected by thromboelastogram, the CD62P value was detected by flow cytometry, and the values were compared. Results: The MA value of frozen platelet thromboelastogram (30.80±6.27 mm) was greater than that of fresh platelets (28.94±5.82 mm), indicating that the coagulation function of frozen platelets in vitro was stronger than that of fresh platelets, while the CD62P content of frozen platelets (29.50±3.06%) was smaller than that of fresh platelets (55.25±4.09%), indicating that the aggregation function of frozen platelets was stronger than that of fresh platelets. Conclusion: Frozen platelets have stronger coagulation function than fresh platelets in vitro. Platelets are stored for a long time after freezing to meet the increasing clinical demand for platelets. |
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| Keywords: | CD62P, cryopreservation, platelet function, thromboelastography | |||||||||||||||||||
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